Many applications in modern chemistry and biochemistry depend on the ability to make, change and analyse proteins and enzymes. After completion of the course students will be familiar with all of the steps required for the production of proteins in bacteria and various techniques of analysis. This includes techniques for making, modifying, and analysing proteins. There is an emphasis on biophysical techniques (SDS-PAGE, light-scattering, CD spectroscopy, ultracentrifugation, mass spectrometry, ITC, fluorescence, surface plasmon resonance), discussing their physical basis in depth. Advanced analysis techniques (X-ray crystallography, NMR, EM, SAXS/SANS) are discussed only superficially. Introduction to bioinformatics: protein sequence alignment, 3D structure analysis, modelling.
Learning Outcomes
Upon successful completion, students will have the knowledge and skills to:
After successful completion of the course students will have:
- Understanding of the necessary elements of protein overexpression systems in bacteria,
- Capability to design all the steps required to produce an expression system for a new protein,
- Capability to make and purify proteins,
- Understanding of techniques for modifying proteins,
- Experience with basic techniques for protein analysis,
- Understanding of advanced biophysical techniques for protein analysis, including the capacity to discuss their relative merits and interpret data from those techniques,
- Familiarity with software for protein visualization, sequence alignment and modelling.
Indicative Assessment
50% by written exam, 50% by weekly assessments of practicals and tutorials (10 in total, 5% each). Students have to pass the exam to pass the course.
In response to COVID-19: Please note that Semester 2 Class Summary information (available under the classes tab) is as up to date as possible. Changes to Class Summaries not captured by this publication will be available to enrolled students via Wattle.
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Workload
The course comprises 26 lectures (1 hour each), 6 practicals (3 hours each) and 8 tutorial/quiz sessions (1 hour each).
Practicals are 3-4 hours each. Each practical requires about 1 hour of preparation and 1 hour of reporting. Assessments require independent reading for about 1 hour per contact hour.
Requisite and Incompatibility
Prescribed Texts
Selected chapters from Biochemistry by Voet & Voet
Specialisations
Fees
Tuition fees are for the academic year indicated at the top of the page.
If you are a domestic graduate coursework or international student you will be required to pay tuition fees. Tuition fees are indexed annually. Further information for domestic and international students about tuition and other fees can be found at Fees.
- Student Contribution Band:
- 2
- Unit value:
- 6 units
If you are an undergraduate student and have been offered a Commonwealth supported place, your fees are set by the Australian Government for each course. At ANU 1 EFTSL is 48 units (normally 8 x 6-unit courses). You can find your student contribution amount for each course at Fees. Where there is a unit range displayed for this course, not all unit options below may be available.
| Units | EFTSL |
|---|---|
| 6.00 | 0.12500 |
Course fees
- Domestic fee paying students
| Year | Fee |
|---|---|
| 2020 | $4050 |
- International fee paying students
| Year | Fee |
|---|---|
| 2020 | $5760 |
Offerings, Dates and Class Summary Links
ANU utilises MyTimetable to enable students to view the timetable for their enrolled courses, browse, then self-allocate to small teaching activities / tutorials so they can better plan their time. Find out more on the Timetable webpage.
Class summaries, if available, can be accessed by clicking on the View link for the relevant class number.
Second Semester
| Class number | Class start date | Last day to enrol | Census date | Class end date | Mode Of Delivery | Class Summary |
|---|---|---|---|---|---|---|
| 8655 | 27 Jul 2020 | 03 Aug 2020 | 31 Aug 2020 | 30 Oct 2020 | In Person | View |